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J Nucl Med. 2011; 52 (Supplement 1):308
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Oncology: Basic, Translational & Therapy: Basic Science

Basic Science IV (Oncology): Novel Image-Based Methods

SSTR2-based reporter imaging in a rabbit VX2 tumor model

Murali Ravoori2, Lin Han2, Sheela Singh2, Katherine Dixon1, Jyoti Duggal2, Rajesh Uthamanthil3, Sanjay Gupta1, Kenneth Wright1 and Vikas Kundra2

1 Department of Diagnostic Radiology (Section of Interventional Radiology), UT MD Anderson Cancer Center, Houston, TX 2 Department of Experimental Diagnostic Imaging, UT MD Anderson Cancer Center, Houston, TX 3 Veterinary Medicine & Surgery, UT MD Anderson Cancer Center, Houston, TX

Abstract No. 308

Objectives: To compare gene expression after intra-arterial and intra-tumoral delivery of adenovirus expressing a SSTR2-based reporter gene in a large animal tumor model as a bridge to clinical translation.

Methods: Nine New Zealand white rabbits bearing VX2 tumors in each thigh were randomly injected either intra-arterially or intra-tumorally with adenovirus containing a human somatostatin receptor type 2A (Ad-CMV-HA-SSTR2A) gene chimera or control adenovirus containing green fluorescent protein (Ad-CMV-GFP). Three days after gene delivery, 111In-octreotide was administered after CT imaging and tumoral uptake of the 111In-octreotide was evaluated the following day using a gamma camera. This procedure was repeated on nine additional rabbits to investigate longitudinal gene expression at five and fourteen days after adenovirus injection. Excised tissue samples were also evaluated to determine octreotide biodistribution. A two-sided student’s paired t test was used to compare transgene activity.

Results: VX2 tumors infected with Ad-CMV-HA-SSTR2 accumulated significantly higher concentrations of radioactivity as compared to tumors infected with the control virus (P<0.05). Intra-arterial and intra-tumoral routes of delivery resulted in similar levels of gene expression. Significantly higher (P<0.05) levels of gene expression were noted early versus 2 weeks after gene delivery.

Conclusions: Transgene expression of SSTR2-based reporters can be visualized after both intra-arterial and intra-tumoral delivery in a large animal tumor model. This is the first SSTR2-based reporter imaging of which we are aware in a large animal model.

Research Support: National Cancer Institute (R21 CA123841-010), John S. Dunn Research Foundation, and NIH Cancer Center Support grant (CA016672)





This Article
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Google Scholar
Right arrow Articles by Ravoori, M.
Right arrow Articles by Kundra, V.
PubMed
Right arrow Articles by Ravoori, M.
Right arrow Articles by Kundra, V.