SNM Annual Meeting Abstracts
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     



J Nucl Med. 2008; 49 (Supplement 1):68P
This Article
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Google Scholar
Right arrow Articles by Li, Z.
Right arrow Articles by Chen, X.
PubMed
Right arrow Articles by Li, Z.
Right arrow Articles by Chen, X.

Novel Approaches to Molecular Imaging

PET/MRI, MRI and Ultrasound

PET/MRI dual modality tumor imaging using RGD conjugated radiolabeled iron oxide nanoparticles

Zibo Li1, Ha-Young Lee1, Kai Chen1, Andrew R Hsu1, Chenjie Xu2, Jin Xie2, Shouheng Sun2 and Xiaoyuan Chen1

1 Stanford University, Stanford, California; 2 Department of Chemistry, Brown University, Providence, Rhode Island


Formula

273

Objectives: The purpose of this study was to develop a bifunctional iron oxide (IO) nanoparticle probe for positron emission tomography (PET) and magnetic resonance (MR) imaging of tumor integrin {alpha}vβ3 expression.

Methods: Poly(aspartic acid)-coated IO nanoparticles (PASP-IO) were synthesized using a co-precipitation method. Particle size and magnetic properties were measured. Phantom study was used to assess the efficacy of PASP-IO as a T2-weighted MR contrast agent. PASP-IO nanoparticles with surface amino groups were coupled to RGD peptides for integrin {alpha}vβ3 targeting and macrocyclic DOTA chelator for PET imaging after labeling with 64Cu. IO nanoparticle conjugates were further tested in vitro and in vivo to determine receptor targeting efficacy and feasibility for dual PET/MR imaging.

Results: PASP-IO nanoparticles made by single-step reaction had a core size of 5-7 nm with a hydrodynamic diameter of ~40 nm. The saturation magnetization of PASP-IO nanoparticles was ~117 emu/g Fe. DOTA-IO-RGD conjugates bound specifically to integrin {alpha}vβ3 in vitro based on cell binding assay. Both microPET and T2Cu-weighted MR imaging showed integrin-specific delivery of conjugated RGD-PASP-IO nanoparticles and prominent reticuloendothelial system (RES) uptake.

Conclusions: We have successfully developed an iron oxide-based nanoprobe for simultaneous dual PET and MR imaging of tumor integrin expression. The success of this bifunctional imaging approach may allow for earlier tumor detection with a high degree of accuracy and provide further insight into the molecular mechanisms of cancer.

Research Support: NCI: R01-CA119053 R21-CA121842 R21-CA102123 P50-CA114747 U54-CA119367 R24-CA93862. DOD: W81XWH-07-1-0374, W81XWH-04-1-0697, W81XWH-06-1-0665, W81XWH-06-1-0042, DAMD17-03-1-0143. MOEHRD: KRF-2006-352-D00061.





This Article
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Google Scholar
Right arrow Articles by Li, Z.
Right arrow Articles by Chen, X.
PubMed
Right arrow Articles by Li, Z.
Right arrow Articles by Chen, X.