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J Nucl Med. 2008; 49 (Supplement 1):66P
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Novel Approaches to Molecular Imaging

Optical and Bioluminescence

Time domain optical imaging of sterile soft tissue abscesses with Cy5.5-scVEGF

Francis Blankenberg1, Zoia Levashova1, Marina Backer2 and Joseph Backer2

1 Radiology, Stanford University, Stanford, California; 2 Sibtech, Inc., Brookfield, California

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Objectives: Time domain optical imaging (TD) uses short pulses of light to excite fluorescent probes such as Cy5.5-scVEGF to generate time-of-flight information to calculate the depth and concentration of tracer in vivo. We now wish to use TD to study the relationship of angiogenesis and soft tissue inflammation using Cy5.5-scVEGF (single chain vascular endothelial growth factor).

Methods: 20 uL of turpentine was injected into the right thigh of anesthesized Balb/c mice. Images were acquired on day 8, 3-3.5 hrs after Cy5.5-scVEGF intravenous injection of probe (10 & 30 ug/mouse). The eXplore Optix system (Advanced Research Technologies) was used to track Cy5.5-scVEGF in vivo. ROIs were obtained from the right (Abs) versus the normal thigh (Bkg) analyzed by OptiView software.

Results: TD of Cy5.5-scVEGF was able to directly visualize right thigh abscesses during the subacute-chronic phases of inflammation (day 8). Interestingly we found that while soft tissue background was relatively unchanged between mice injected with 10 and 30 ug of probe that Abs/Bkg was significantly higher with the larger dose (7.95 vs. 4.37, respectively).

Conclusions: TD with Cy5.5-labeled scVEGF can be used to monitor subacute-chronic soft tissue abscesses with higher Abs/Bkg ratios achieved with 30 vs. 10 ug per mouse. Additional time course studies with Cy5.5-scVEGF and other inflammatory related probes (Cy5.5-annexin V-128) are ongoing.

Research Support: 1 R43 EB006679-01A2 (to JB and FG)


Figure 1
Table I





This Article
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Right arrow Articles by Backer, J.