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J Nucl Med. 2008; 49 (Supplement 1):50P
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Oncology-Basic Science: Basic Science

Imaging - Peptides and Small Molecules

A comparative study of n.c.a. sodium [18F]fluoroacetate and sodium [11C]acetate in xenotransplanted tumor bearing mice

Ralf Bergmann1, Susan Richter1, Jens Pietzsch1, Bettina Beuthien-Baumann2 and Frank Wuest1

1 Radiopharmaceutical Biology, Forschungszentrum Dresden, Dresden, Germany; 2 Department of Nuclear Medicine/PET Centre Rossendorf, University Hospital Carl Gustav Carus, Dresden, Germany

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Objectives: This study describes the radiosynthesis of [18F]fluoroacetate (18F-FAC), radiopharmacological characterization, and molecular imaging of oxidative metabolism in tumor bearing mice using 18F-FAC in comparison with 11C-ACE.

Methods: The radiosynthesis of n.c.a. sodium 18F-FAC was performed in two step reaction sequence and subsequent SPE purification in a remotely controlled synthesis module. Biodistribution, metabolism and small animal PET studies of 18F-FAC and 11C-ACE were carried out in rats and HT-29 tumor-bearing mice.

Results: 18F-FAC was obtained in radiochemical yields of 20-25% within 50 min. Biodistribution data showed higher initial radioactivity uptake in most organs and tissues for 18F-FAC; the initial brain uptake of 0.67 %ID/g at 5 min p.i. followed by a 22% clearance at 60 min p.i. Both radiotracers can clearly delineate the tumor. The tumor-to-muscle ration was 1.8 for 18F-FAC and 1.5 for 11C-ACE. Unlike 11C-ACE, 18F-FAC shows a slow transport of the free radiotracer from the blood pool into the tumor, and 10% of the free fraction of 18F-FAC is trapped in tumor tissue.

Conclusions: The highly reproducible remotely-controlled two step/one pot synthesis of 18F-FAC represents an alternative to previously published synthesis routes. The successful PET imaging of xenotransplanted human colorectal adenocarcinoma tumor HT-29 by means of 18F-FAC shows that the radiotracer may not only be restricted for imaging of previously reported prostate cancer tumors. However, the exact mechanism of 18F-FAC tissue uptake remains unclear and should be subject of further studies.

Research Support: Supported in part by the EU FP6 ‘‘BioCare’’, proposal #505785.





This Article
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Right arrow Alert me to new issues of the journal
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Right arrow Articles by Bergmann, R.
Right arrow Articles by Wuest, F.
PubMed
Right arrow Articles by Bergmann, R.
Right arrow Articles by Wuest, F.