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J Nucl Med. 2008; 49 (Supplement 1):432P
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Technologist Student Abstracts

Technologist Student Papers III

Myocardial perfusion imaging in mice using [99mTc]-sestamibi pinhole SPECT

Sebastian Nowak1, Christoph Rischpler2, Christian Zach1, Peter Bartenstein1 and Marcus Hacker1

1 Department of Nuclear Medicine; 2 Department of Cardiology, University of Munich, Munich, Germany

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Objectives: Myocardial perfusion imaging can be used to provide quantitative measurements of myocardial infarction, a surrogate endpoint for monitoring stem cell therapy. Aim of the present study was to establish a pinholeSPECT system for acquisition and evaluation of perfusion studies in mice.

Methods: 370 Mbq [99m Tc]-sestamibi were injected into a tail vein of C57BL/6J wildtype mice. Images were aquired in SPECT technique starting 45 min p.i. using a triple-headed gamma camera, each detector head equipped with a custom made pinhole collimator. All images were reconstructed applying 6 iterations of an iterative row action maximum likelihood algorithm. The radius of rotation was set to 4 cm (magnification 4). Multiple image acquisitions were performed varying the following presets and reconstruction parameters: pinhole diameter, number of projection angles, time per projection, image matrix and zoom factor.

Results: Center of rotation error was corrected by scanning a multiple point phantom and iteratively adjusting the center-of-rotation offsets. The same source was used to measure the spatial resolution of the system for 99mTc. Optimal image quality was achieved using a 0.5 mm pinhole diameter, acquiring in a 128 x 128 x 128 matrix with an isotropic voxel size of 0.445 mm zoom factor of 2 using 20 projection angles (6° per step, 120° for each head), 90 seconds each, resulting in a total acquisition time of 30 min. Applying these parameters a resolution of 1.6 mm with a pixel size of 0.556 mm could be achieved.

Conclusions: Myocardial perfusion imaging in mice is feasible with good image using a triple headed gamma camera, each detector equipped with a pinhole SPECT collimator, leading to a resolution of 1.6 mm with a pixel size of 0.556 mm.





This Article
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Google Scholar
Right arrow Articles by Nowak, S.
Right arrow Articles by Hacker, M.
PubMed
Right arrow Articles by Nowak, S.
Right arrow Articles by Hacker, M.