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J Nucl Med. 2008; 49 (Supplement 1):353P
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Oncology-Clinical Diagnosis: Solid Tumors

Clinical Diagnosis-Solid Tumors Posters

18F-Fluorothymidine PET/CT in ovarian carcinoma

Badreddine Bencherif1, Julie Deloia2, Scott Richard2, Robert Edwards2, Esther Elishaev3, Scott Mason1, Dilip Shinde4, Daniel Pryma1, Ashok Muthukrishnan1 and James Mountz1

1 Radiology, UPMC Presbyterian, Pittsburgh, Pennsylvania; 2 Obstetric-Gynecology; 3 Pathology; 4 Radiology, UPMC Magee Women's Hospital, Pittsburgh, Pennsylvania

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Objectives: To assess in patients with ovarian carcinoma, the relationship between maximum standardized uptake values (SUV) of 3'deoxy-3'-[18F] fluorothymidine (18F-FLT), an indicator of tumor cell proliferation, and in vitro quantitative pathologic measures of cellular proliferation.

Methods: Six female patients (61+/-4.8 years,58-70 years) with suspected or known ovarian cancer underwent a combined PET/CT, sixty minutes post-intravenous administration of 185 MBq of 18F-FLT. Images from pelvis to neck were obtained on a GE Discovery ST scanner and the SUV of PET positive and control areas were obtained. A full abdominal surgical exploration with complete cytoreduction was then performed. When not feasible PET positive and control sites were biopsied and tissue collected for in vitro studies. Tissue was divided and used for ki-67 proliferation index staining, RNA isolation for rt-PCR for thymidine kinase-1 (TK1) levels, and grown ex vivo for DNA cell proliferation analysis. Univariate analysis was performed using the paired student’s t-test.

Results: PET positive lesions were found to have significantly increased ki-67 when compared to control lesions (12.8 vs. 0.4, P=0.02) but there was no differences in relative TK1 levels (P=0.10) or ex vivo cell proliferation ability (P=0.30). A significant positive correlation between SUV and ki-67 was found (R2=0.68, P=0.04) while a trend (R2=0.60, P=0.07) was seen with CA-125.

Conclusions: In malignant ovarian cells, increased FLT activity by PET scan correlates with ki-67 index, but not with TK1 levels or DNA content.

Research Support: Supported by a grant from The Myrtle Forsha Foundation.





This Article
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Right arrow Email this article to a friend
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Right arrow Alert me to new issues of the journal
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Right arrow Articles by Bencherif, B.
Right arrow Articles by Mountz, J.
PubMed
Right arrow Articles by Bencherif, B.
Right arrow Articles by Mountz, J.