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J Nucl Med. 2008; 49 (Supplement 1):334P
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Oncology-Basic Science: Therapy, Metrics & Intervention

Therapy, Metrics & Intervention Posters

Small animal PET for the serial evaluation of lung squamous cells carcinoma xenograft in nude mice

Valentina Ambrosini1, Carmelo Quarta1, Milena Fini2, Cristina Nanni1, Deborah Malvi3, Domenico Rubello4, Silvia Trespidi1, Antonello Spinelli1, Roberto Franchi1 and Stefano Fanti1

1 Nuclear Medicine, S. Orsola-Malpighi Hospital, Bologna, Italy; 2 Experimental Surgery, Isituti Ortopedici Rizzoli, Bologna, Italy; 3 Pathology, S. Orsola-Malpighi Hospital, Bologna, Italy; 4 Nuclear Medicine, S. Maria della Misericordia Hospital, Rovigo, Italy

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Objectives: To monitor human lung non-small cells carcinoma xenograft growth in nu/nu mice by 18F-FDG Small Animal PET.

Methods: Human cell line HCC15 (lung squamous cells carcinoma) was subcutaneously inoculated (107cells/mouse in 100ul PBS) in seven 6weeks-old nu/nu mice at right flank level. Tumour growth was serially monitored by 18F-FDG Small Animal PET (18F-FDG i.v. injected dose: 20MBq in <0,15ml; uptake time=60minutes, 15minutes static acquisition of 1 bed position) at 7, 14, 21 and 35 days after cells injection. PET scans were performed under gas (Sevofluorane 5%, Oxygen 1L/min) anaesthesia. FDG-PET images were reconstructed iteratively (OSEM 2D) and read in three planes. PET was considered positive if any area of increased non-physiologic FDG uptake was observed. Semi-quantitative analysis was performed using the TBR (max counts in target ROI/mean counts in the background). Tumour volume was measured by caliper and calculated as major diameter x minor diameter2/2. Tumour burden was calculated for each scan as mean TBR x mean tumour volume.

Results: PET was positive at 7 days in 2 animals and at 14 days in 4. TBR showed a mild decrease over time while tumour volume rapidly increased (figure 1). The tumour burden progressively increased (figure 2).

Conclusions: Small animal PET detected xenograft tumours 7 days after implantation, thus earlier then macroscopic evaluation. The trend of the TBR and tumour volume suggests to perform additional PET scans before 7 days and between 7 and 14 days in order to detect the smallest tumour lesion.





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Right arrow Articles by Fanti, S.
PubMed
Right arrow Articles by Ambrosini, V.
Right arrow Articles by Fanti, S.