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This Article Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Google Scholar Articles by Carter, E. Articles by Fischman, A. PubMed Articles by Carter, E. Articles by Fischman, A.

Hind limb burn injury induces systemic changes in glucose metabolism

¹ Nuclear Medicine; ² Surgery, Massachusetts General Hospital & Shriners Hospital for Children, Boston, Massachusetts

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Objectives: It has been have suggested that burn trauma to the extremities produces systemic effects. Recently, we demonstrated that burn injury is associated with changes in FDG uptake by brown fat (JNM 2007;48:270P). In this study we examined the effects of hindlimb burn injury on systemic glucose metabolism.

Methods: Male mice (28 grams) were anesthetized, both hind-limbs were shaven, and the right limb was subjected to scald injury in 90^oC water for 5 sec. The animals were placed in wire bottom cages with no food and free access to water. 24 h later, the mice were injected with 5 µCi of FDG. One hour later, the mice were euthanized, and biodistribution measured. Sham treated animals served as controls. The results were expressed as %ID/g (mean ± sem).

Results: The scald procedure produced a full skin thickness injury with sharp margins. Compared with sham mice, FDG uptake was dramatically elevated in brown fat of scalded animals (42.68 ± 14.1 vs 9.88 ± 1.1; p<0.001). In contrast, FDG uptake by skeletal muscle of the scalded leg (outside of the scald region) and contralateral uninjured limb was significantly reduced compared with sham treated animals (2.37 ± 0.7 vs 5.67 ± 1.1 ; p<0.01 and 4.60 ± 0.8 vs 5.67 ± 1.1; p<0.05). FDG uptake was also significantly reduced in spleen (p<0.05), adrenal gland (p<0.05), GI tract (p<0.05), testes (p<0.05), bone (p<0.05) and brain (p<0.01) of injured animals.

Conclusions: Burn injury to a single leg resulted in a massive elevation of FDG uptake by brown fat but reduced uptake in other tissues; including brain. These findings are consistent with the hypothesis that burn trauma to the extremities can produce marked systemic effects.

Research Support: NIH & Shriners Hospitals for Children

This Article Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Google Scholar Articles by Carter, E. Articles by Fischman, A. PubMed Articles by Carter, E. Articles by Fischman, A.