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[14C]-Choline autoradiography of prostate cancer: Effects of castration and uptake time interval

¹ Department of Radiology, University of Southern California, Los Angeles, California

1393

Objectives: To investigate the effects of castration and uptake time interval on the level of [14C]-choline accumulation in murine-implanted human prostate tumor xenografts using quantitative autoradiography.

Methods: We implanted CWR22 and PC3 human prostate cancer cells in castrated (n=9) and non-castrated (n=9) athymic male mice and allowed tumors to grow to 1 cm. The mice were euthanized at 5, 10 and 20 min after injection of 5 µCi [14C]-choline. Mice were prepared for quantitative autoradiography with DLU of viable tumor sections (range 9-18 samples/time point) converted to units of radioactivity (nCi/mm2) using calibration. Two-group comparisons were performed using a two-tailed Student t test with unequal variance and with significance probability level of less than 0.05.

Results: The results are summarized in the following 2 graphs. Two-group comparisons between the means of the tracer uptake level for each tumor type at each of 3 time points for each of 2 host types showed that: 1) level of tracer localization in the two tumor types was affected little in relation to the host type, and 2) PC3 tumor uptake level tended to increase slowly with time only in the non-castrated host while this was not observed in the castrated host or with CWR22 tumor in either host type.

Conclusions: The uptake time interval and castration do not significantly affect the level of [14C]-choline uptake by the human prostate cancer xenograft. Our results suggest that [11C]-choline PET may be performed early after tracer administration and that androgen ablation may not adversely affect the lesion detection sensitivity. Our animal-based results are consistent with the recent preliminary human-based observations (Krause B et al. Eur J Nucl Med Mol Imaging 35:18-23, 2008).

Research Support: Supported by NIH/NCI R01-CA111613-01.

This Article Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Google Scholar Articles by Jadvar, H. Articles by Conti, P. PubMed Articles by Jadvar, H. Articles by Conti, P.