SNM Annual Meeting Abstracts
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     



J Nucl Med. 2008; 49 (Supplement 1):300P
This Article
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Google Scholar
Right arrow Articles by Jeon, H. R.
Right arrow Articles by Yoo, J.
PubMed
Right arrow Articles by Jeon, H. R.
Right arrow Articles by Yoo, J.

Radiopharmaceutical Chemistry: New Chemistry-Other

New Chemistry-Other Posters

Synthesis of oleyl-4-[131I]-iodobenzoate as long term cell trafficking agent

Hak Rim Jeon1, Young Jin Lee2, Saengmyung Han1, You La Lee2, Wonjung Kwak1, Darpan Pandya1, Sang-Woo Lee2, Byeong-Cheol Ahn2, Jaetae Lee2 and Jeongsoo Yoo1

1 Molecular Medicine and Nuclear Medicine; 2 Nuclear Medicine, Kyungpook National University, Dae-gu, South Korea

1279

Objectives: Labeling with F-18 (t1/2=110min) and Cu-64 (t1/2=12.7h) has been most devoted for monitoring movement of cells over a time course. However, these radionuclides do not have sufficiently long half-life for long-term cell trafficking study. Here we report a preparation of long term cell trafficking agent with I-131 (t1/2)=8d. This agent was used to radiolabel different cells by absorption into cell membranes.

Methods: Treatment of 4-iodobenzoyl chloride with oleyl alcohol was yielded oleyl-4-iodobenzoate, followed by nucleophilic aromatic substitution with hexabutyl tin. Oleyl-4-(tributylstannyl) benzoate was treated with 3% H2O2, 1M HCl, and [131I]NaI to give oleyl-4-[131I]-iodobenzoate ([131I]OIB). Cell labeling was accomplished by simply incubation of [131I]OIB and cells in PBS, followed by successive washing and isolation of the cells by centrifugation. Over 2 weeks, gamma camera images were measured after myocardium injection of the [131I]OIB-labeled cells.

Results: ([131I]OIB)was prepared in three steps efficiently. The radiochemical yield of [131I]OIB was higher than 50%. The radiolabeled [131I]OIB was obtained in high radiochemical purity (> 93%) after HPLC purification. The yield of cell labeling of [131I]OIB with L6, H9C2, and Hela cells was 32±6.9%,27±5.2%, and 22±6.7%, respectively. Gamma camera imaging of implanted [131I]OIB-labeled L6 cells showed high retention in rat heart up to 2 weeks.

Conclusions: [131I]OIB was simple to prepare and cells were labeled effectively. [131I]OIB and its other radioiodine analogs, [124/125I]OIB are expected to play a role as a long term cell trafficking agent.

Research Support: This work was supported by the Brain Korea 21 Project in 2008.





This Article
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Google Scholar
Right arrow Articles by Jeon, H. R.
Right arrow Articles by Yoo, J.
PubMed
Right arrow Articles by Jeon, H. R.
Right arrow Articles by Yoo, J.