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J Nucl Med. 2008; 49 (Supplement 1):299P
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Radiopharmaceutical Chemistry: New Chemistry-Other

New Chemistry-Other Posters

Visualization of extrahepatic rodent islet transplants with [C-11]DTBZ

J. S. Dileep Kumar1, Paul Harris3, Hugo Sondermeijer4, Chitra Saxena2, David Woodland4, Abbas Rana4, Qin Rao2, Mark Hardy3 and Piotr Witkowski4

1 Psychiatry and Radiology; 2 Radiology; 3 Medicine; 4 Surgery, Columbia University Medical Center, New York, New York

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Objectives: In the treatment of diabetes, islet transplantation (iTx) to the liver reestablishes normal feedback regulation of insulin secretion and normoglycemia. The Edmonton iTx protocol is associated with good short-term success but only a 10-15% success rate by 5 years post-iTx. Understanding islet graft failure and a non invasive method to estimate transplanted β-cell mass seems prerequisite before iTx outcomes improve. β-cell mass (BCM) measurements by PET with [11C] DTBZ is not suitable for islets transplanted to the liver due to catabolism of the radioligand. Here we show feasibility of estimating BCM in iTx to an extrahepatic site- the intramuscular space of the abdominal wall prevascualrized with alginate scaffold containing RGD peptide and vascular growth factors.

Methods: Normal Lewis rats were made diabetic with streptozotocin and then transplanted with 3000 purified allogeneic ACI islets. ITx reversed diabetes by day 2 and the abdomen was imaged (90 min) dynamically using 250 +/-50 µCi [11C]DTBZ and a Concorde microPET scanner on day six post Tx.

Results: In transplanted rodents without reversal of hyperglycemia and sham operated rodents, no preperitoneal uptake of radioligand was demonstrated. In diabetic rodents with euglycemia restored by iTx, an intramuscular islet cell mass is clearly revealed by uptake of [11C]DTBZ.

Conclusions: For islets transplanted to non hepatic sites, PET scans with [11C]DTBZ may offer a means to monitor islet graft function and survival.





This Article
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Right arrow Alert me to new issues of the journal
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Right arrow Articles by Kumar, J. S. D.
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PubMed
Right arrow Articles by Kumar, J. S. D.
Right arrow Articles by Witkowski, P.