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J Nucl Med. 2008; 49 (Supplement 1):28P
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Cardiovascular: Basic Science

Molecular Targeting - Plaques and Stem Cells

Direct radiolabeling of cardiac derived stem cells by 18FDG for quantitation of cell retention early after intramyocardial injection

John Terrovitis1, Riikka Lautamäki1, Keng Fai Kwok1, James Engles1, Jurgen Seidel1, Martin Pomper1, Benjamin Tsui1, M.Roselle Abraham1, Eduardo Marbán1 and Frank Bengel1

1 Johns Hopkins University, Baltimore, Maryland

110

Objectives: Stem cell transplantation is emerging as a promising treatment option for heart failure and cardiac derived stem cells (CDCs) may be the cell of choice for this purpose. In order to optimize cell delivery and early engraftment, we sought to establish a method for direct CDC labeling using 18FDG. We hypothesized that this would enable quantification of cell retention in vivo after direct intramyocardial injection by PET.

Methods: Maximal nontoxic 18FDG dose and optimal labeling time point/conditions were determined in vitro. For in vivo imaging, 2x106 rat CDCs were incubated with 2µCi of 18FDG /mL of media for 30min. Eight syngeneic Wistar Kyoto rats underwent left coronary artery ligation and cells were injected directly into the infarct area. The 18FDG PET images reconstructed from the acquired data were coregistered with the subsequently acquired myocardial perfusion 13NH3 PET images. To determine the influence of CDC viability, a similar number of lysed labeled rCDCs was injected in two infarcted animals.

Results: When non viable (lysed) CDCs were injected, only 2.1±1.3% of the injected activity was retained in the heart, indicating that there is very low reuptake of released radiolabel by the myocardium. In animals that received viable CDCs, retention at 1 hour postinjection was 20.8±8.3%, a low rate that is in accordance with previous studies that quantified efficiency of cell delivery by ex vivo methods.

Conclusions: Radiolabeling of cardiac stem cells with 18FDG allows for robust visualization of injected cells in vivo and quantitation of short term cell engraftment by PET. This method will facilitate the development of techniques to enhance cell engraftment in the future.

Research Support: Donald W. Reynolds Foundation and NHLBI





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