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This Article Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Google Scholar Articles by Cho, S. Articles by Wahl, R. PubMed Articles by Cho, S. Articles by Wahl, R.

AMP and FDG lung uptake in a mouse model of cystic fibrosis

¹ Division of Nuclear Medicine, Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins School of Medicine, Baltimore, Maryland; ² Pulmonary Division, Department of Pediatrics, Johns Hopkins University, Baltimore, Maryland

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Objectives: We previously showed high normal lung uptake of ³H-adenosine 5’-monophosphate (AMP). It has been reported that cystic fibrosis transmembrane conductance regulator (CFTR) is involved in ATP transport and extracellular adenylates are dysregulated in cystic fibrosis (CF). Our goal in this study was to evaluate lung uptake of AMP and ¹⁸F-FDG (FDG) using a mouse model of CF.

Methods: CFTR knockout mice (CF) and age- and sex-matched C57BL/6 mice (WT) were used as controls. Mice were exposed to 10µg/50µl Pseudomonas Aeruginosa LPS (n=3 or 4) by intubation and direct intrapulmonary injection. Control mice underwent the same procedure but received with 50µl of PBS (n=3). Two days later, the mice were co-injected intravenously with 37kBq (1µCi) of AMP and 3.7MBq (100 µCi) of FDG. After 1 hour the mice were euthanized for ex vivo biodistribution.

Results: Ex vivo biodistribution tissue uptake values are expressed as average of percent total injected dose activity/gram of tissue (%ID/g) and blood normalized values (ratio of %ID/g of organ/ blood). In mice exposed to PBS alone there was higher uptake of AMP and FDG in CF mice compared to WT which had borderline statistical significance (FDG P=0.027; AMP P=0.058); however normalization for blood activity did not reach statistical significance. No significant difference in level of AMP or FDG uptake was observed in the CF compared to WT mice with LPS lung inflammation.

Conclusions: There was increased lung uptake of FDG and AMP in CF versus WT mice, which may be biologically significant and requires further evaluation. No significant difference in AMP or FDG lung uptake was seen in CF and WT mice associated acute inflammation.

This Article Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Google Scholar Articles by Cho, S. Articles by Wahl, R. PubMed Articles by Cho, S. Articles by Wahl, R.