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J Nucl Med. 2008; 49 (Supplement 1):267P
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General Clinical Specialties: Musculoskeletal

Musculoskeletal Posters

DEXA analysis on the bones of rats: Reproducibility of repeated measurements with or without repositioning for various skeletal sites

Dong Hyun Oh1, Gi Jeong Cheon1, Jung Sub Lim2, Yoon Sin Oh3, Sang Chul Park3, Chang Woon Choi1 and Sang Moo Lim1

1 Nuclear Medicine; 2 Paediatrics, Korea Institute of Radiological and Medical Sciences, Seoul, South Korea; 3 Department of Biochemistry and Molecular Biology, Seoul National University, Seoul, South Korea

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Objectives: The measurements of DEXA need to be precise and sensitive enough to be capable of detecting small changes in bone mass of rats. Using a regular Dual-eneragy x-ray absorptiometry, we measured many BMD of various skeletal sites in rats to examine reproducibility of DEXA analysis on the bones of rats.

Methods: We used 12 male rats. Using a DEXA and small animal software(DEXA; Prodigy, General-electric Lunar), scans were performed 4 times in all 12 rats on the same day without repositioning. Another four scans for 6 of these rats were done with repositioning between scans. Customized ROIs, encapsuate the right hind limb, L-spine, skull and pelvic bones were drawn at each measurement. The reproducibility of the measurements was evaluated by measuring the CV of four measurements of BMD at each skeletal sites of all rats and another four measurements with repositioning for six rats. The data were analyzed using Prism 5.0(GraphPad software, Inc).

Results: CVs obtained at different skeletal sites of all measurements without and with repositioning. it was 3.32% and 2.62% for the hind limb, 3.36% and 4.58% for the lumbar spine, 0.80% and 1.53% for skull and 2.85% and 1.45% for pelvic bones, respectively.

Conclusions: Our findings indicate that DEXA is a very reliable instrument on rats regardless of repositioning. The somewhat lower reproducibility of BMD measurements with repositioning at the lumbar spine and skull compared with BMD measurements without repositioning is probably related to change of degrees of flexion of lumbar spine.





This Article
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Right arrow Articles by Oh, D. H.
Right arrow Articles by Lim, S. M.
PubMed
Right arrow Articles by Oh, D. H.
Right arrow Articles by Lim, S. M.