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J Nucl Med. 2008; 49 (Supplement 1):245P
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General Clinical Specialties: Gastroenterology

Gastroenterology Posters

Radiolabeling human hepatocytes in order to determine the biodistribution of the transplanted cells

Kuldeep Bhargava1, Daniel Benten2, Brigid Joseph2, Mari Inada2, Kang Cheng2, Charito Love1, Christopher Palestro1 and Sanjeev Gupta2

1 Nuclear Medicine and Molecular Imaging, North Shore-Long Island Jewish Health System, New Hyde Park, New York; 2 Marion Bessin Liver Research Center, Albert Einstein College of Medicine, Bronx, New York

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Objectives: To investigate the feasibility of radiolabeling human hepatocytes (HH) for the purpose of assessing the biodistribution of transplanted hepatocytes.

Methods: Fetal (F) & adult (A) HH were incubated with 370-555 MBq 99mTc using UltraTag® or Ceretec®, or with 15 MBq 111In-oxine. Cell viability was determined by trypan blue exclusion. Labeled HH were transplanted by intraportal or intrasplenic injection in 8 groups of NOD/SCID mice (n=3-5/group). Liver, spleen, lungs, kidneys & bowel were excised 1 hr. post injection & the percent injected dose in theses organs was determined. The radionuclide determined biodistributions of the transplanted HH were verified with DNA PCR & in situ hybridization with human-specific molecular probes.

Results: Neither FHH nor AHH incorporated 99mTc UltraTag® or Ceretec®. Using 111In-oxine, the labeling efficiency was 51±9% for FHH & 78±2% for AHH (p=ns). HH viability before labeling was 84±9%; after labeling viability was 78±12% (p=ns). 94±1% of FHH injected intraportally were in the liver vs 51±15% of FHH injected intrasplenically (p=0.002). Transplanted AHH showed similar results. The radionuclide determined biodistribution data showed good correlation with molecular assays.

Conclusions: (1) Fetal & adult human hepatocytes can be labeled with 111In-oxine but not with 99mTc UltraTag® or Ceretec®. (2)The biodistribution of transplanted human hepatocytes can be determined accurately with 111In-labeled HH. Radionuclide studies may be useful for liver directed hepatocyte therapy.

Research Support: Funded in part by NIH R01-DK-46952 & P30-DK-41296.





This Article
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Right arrow Articles by Bhargava, K.
Right arrow Articles by Gupta, S.
PubMed
Right arrow Articles by Bhargava, K.
Right arrow Articles by Gupta, S.