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J Nucl Med. 2008; 49 (Supplement 1):212P
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Neurosciences: Basic Science

Basic Science Posters

Relative cerebral perfusion in rat using microPET analysis following [11C]butanol injection

Walter Zink1, Michael Synan1, Paresh Kothari1, Shankar Vallabhajosula1 and Stanley Goldsmith1

1 Radiology, Citigroup Biomedical Imaging Center (CBIC), Weill Cornell Medical College, Cornell University, New York, New York

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Objectives: Development of quantitative techniques to determine regional cerebral blood flow/perfusion in rodent models is essential for the evaluation of therapeutic strategies. We report here initial studies of brain perfusion studies using MicroPET and [11C]butanol in a rat model of cerebral artery occlusion.

Methods: A femoral artery-vein fistula (AVF) was created in three rats under isofluorane anesthesia prior to MicroPET (Focus 220) imaging with brain and aortic arch included in the FOV. The extra-corporeal AVF was placed in a shielded coincidence detector outside the MicroPET scanner. PET images and blood-time activity measurements were acquired for 60 s following tail vein injection of 0.8-1.2 mCi [11C]butanol. Subsequent animals were subjected to two-hour MCAO using an endoluminal filament technique. Experimental animals were placed in the microPET ninety minutes after MCAO. Images and time-activity curves were acquired, as above. Twenty-four hours later T2-FLAIR MRI was performed at 3 Tesla. PET and MR images were fused using PMOD software and the infarct zone identified by high T2-FLAIR signal. Relative cerebral blood flow was calculated from ROIs within the infarct zone and contralateral hemisphere using PMOD.

Results: Flow within the infarction zone was significantly reduced compared to the contralateral hemisphere in 3 rats with MCAO. There was a reproducible relationship in the time-activity profiles of aortic arch and extra-corporeal AVF.

Conclusions: [11C]butanol microPET can detect relative cerebral blood flow differences in a rat model of MCAO. Time-activity data from the aortic arch represents a valid arterial input function. Attempts to extract absolute cerebral blood flow values in this model are ongoing.





This Article
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Right arrow Articles by Goldsmith, S.
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Right arrow Articles by Goldsmith, S.