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Neurosciences: Basic ScienceBasic Science Posters |
1 Department of Nuclear Medicine; 2 Eye and ENT Hospital, Fudan University, Shanghai, Shanghai, China; 3 Advanced Detection Equipment Research Center; 4 Radiopharmaceutical Research Centre, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, Shanghai, China
892
Objectives: To investigate the distribution, changes and a possible role of dopamine transporter (DAT) using 99mTc-TRODAT-1 in form deprivation myopia retina.
Methods: guinea pigs were randomly assigned into two groups: form deprivation myopia (FDM) group and normal control group . The test group wore a translucent goggle covering randomly for 4 weeks, and both groups underwent biometric measurement before and after the experiment. At the 4th week, all the guinea pigs were performed both Micro-SPECT imaging and ex vivo autoradiography with injection of 99mTc-TRODAT-1.
Results: In Micro-SPECT imaging, the ratio of 99mTc-TRODAT-1 uptake in the myopic retina (11.55±2.80) was 3.64±1.40 lower than that in the control eye (15.20±1.98, P = 0.026, F = 2.94, t = 2.605), and 2.35±1.05 lower than that in the fellow eyes (13.90±2.04, P = 0.003, t = 5.476). In ex vivo autoradiography, the ratio of 99mTc-TRODAT-1 uptake in the myopic retina (95.52±12.04) was 18.54±5.86 lower than that in the control eye (114.06±7.81, P = 0.01, F = 0.331, t = 3.164), and was 16.95±5.78 lower than that in the fellow eyes (112.47±15.67, P = 0.001, t = 7.179). All the differences were statistically significant, and the results of Micro-SPECT were consistent with those of ex vivo autoradiography.
Conclusions: This study results demonstrated that 99mTc-TRODAT-1 can be used to trace the distribution and changes of DAT in retina. Radionuclide tracing may provide a new approach in vivo for further studies on the dopamine system in the myopia.
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