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J Nucl Med. 2008; 49 (Supplement 1):185P
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Cardiovascular: Basic Science

Basic Science Posters

Quantitative regional myocardial blood flow and coronary flow reserve in conscious rats assessed using split-dose 201Tl and a dedicated dynamic micro-SPECT camera

Teramoto Noboru1, Zeniya Tsutomu1, Mitsuyuki Ose1, Hiroshi Watabe1, Kazuto Fukushima1, Asako Takeuchi1 and Iida Hidehiro1

1 National Cardiovascular Center, Suitua City, Osaka, Japan

806

Objectives: 201Tl has a potential for absolute quantitation of myocardial blood flow (MBF) and coronary flow reserve (CFR) in vivo in small animals. This study was intended to measure absolute MBF and CFR in non-anesthetized rats using a dedicated pinhole-SPECT camera with i.v. split-dose 201Tl. Feasibility of our A-V shunt-based arterial input function monitoring system was also evaluated.

Methods: Experiments were carried out on 16 un-anesthetized rats. 12 were normal and 4 with heart failure induced with high-dose saline. A dynamic scan was carried out with split-dose 201Tl at an interval of 30 min. A catheter was placed to shunt the femoral artery to vein, and the radioactivity in the tube was continuously monitored using a GSO-detector assembly. An adenosine A2A-selective agonist, CGS-21680, was injected at 10 min before the 2nd 201Tl. Rest-and increased MBF’s were calculated by NLLSF, and investigated were the dose dependency of MBF and alterations in a diseased model.

Results: Tissue radioactivity curves showed enhanced rise and clearance after 2nd 201Tl, dependent on the dose of CGS, suggesting dose dependent MBF and CFR. The rise and clearance were reduced in diseased model both at rest and after CGS, suggesting reduced resting MBF and CFR. Arterial input function was well assessed, and SCM fit can be applied. MBF was 2.99+/-0.23 mL/min/g at rest and 4.2+/-0.67 mL/min/g after CGS in normal with maximum dose of CGS(0.02mg/kg), while 1.99+/-0.34 mL/min/g at rest and 3.0+/-0.49 mL/min/g after CGS in diseased model.

Conclusions: Quantitation of MBF and CFR can be achieved using 201Tl and dynamic micro-SPECT in rats. Arterial input function can be monitored with minimal effects of physiological condition.





This Article
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Right arrow Alert me to new issues of the journal
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Google Scholar
Right arrow Articles by Noboru, T.
Right arrow Articles by Hidehiro, I.
PubMed
Right arrow Articles by Noboru, T.
Right arrow Articles by Hidehiro, I.