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J Nucl Med. 2008; 49 (Supplement 1):182P
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Cardiovascular: Basic Science

Basic Science Posters

Tracking of mesenchymal stem cells via three transplantation routes in rats with myocardial infarction

Harrison Barrett1, Zhonglin Liu1, Lars Furenlid1, James Woolfenden1, Mary Murphy2, Cynthia Coleman2 and Frank Barry2

1 University of Arizona, Tucson, Arizona; 2 National University of Ireland Galway, Galway, Ireland

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Objectives: Mesenchymal stem cells (MSCs) have shown promising results in repairing the injured heart. However, many aspects of the delivery protocol will critically influence the outcome. The transplantation route is of particular importance as it will have significant impact on the fate of the transplanted cells. The aim of this study was to assess myocardial homing of transplanted 111In-labeled MSCs via 3 delivery routes in rats.

Methods: MSCs were isolated from the tibia and fibula of 8-week old Fischer rats and labeled with 111In-oxine. 111In-MSCs were respectively administered via intravenous (IV, n=4), intracoronary (IC, n=3), and intramyocardial injection (IM, n=4) in adult rats with acute myocardial infarction. Three normal rats received 111In-MSCs via IV injection. SPECT images were acquired from 1 hr up to 6 days after 111In-MSCs injection. Dual-isotope imaging of 111In-MSCs/99mTc-sestamibi was performed within 24-48 hrs.

Results: Cell labeling efficiency was 61%-70%. When MSCs were given by IV route, a high lung uptake was observed 1-6 hrs post-injection. At 24-hr, the initial lung activity had shifted toward other organs. 111In remained locally detectable in infarcted hearts within 6 days, but not in the healthy hearts. The overall activity detected in the hearts which received 111In-MSCs via IM and IC route was significantly higher than that by IV delivery. The activities in IC hearts were more widely distributed in the ischemic areas compared to that in the IM hearts, which accumulated in the injection sites.

Conclusions: Myocardial homing of intravenously introduced MSCs was demonstrated in the rat hearts with infarcts. IC delivery of MSCs may offer advantages over direct myocardial injection in terms of cell-homing and engraftment.





This Article
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Right arrow Email this article to a friend
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Right arrow Alert me to new issues of the journal
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Right arrow Articles by Barrett, H.
Right arrow Articles by Barry, F.
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Right arrow Articles by Barry, F.