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J Nucl Med. 2008; 49 (Supplement 1):165P
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Novel Approaches to Molecular Imaging

Novel Approaches to Molecular Imaging Posters

Parkinson’s disease: Role of molecular imaging in monitoring the effectiveness of stem cell therapy

Mathew Thakur1, Zixiong Cao1, Ming Yang2, Kaijun Zhang1, Angela Donaldson2, Richard Freifelder3, Christopher Cardi1, Dutta Ponde3, Boby Mathews3 and Lorraine Iacovitti2

1 Radiology; 2 Neurology, Thomas Jefferson University, Philadelphia, Pennsylvania; 3 Radiology, University of Pennsylvania, Philadelphia, Pennsylvania

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Objectives: The goal of this study was to monitor the effectiveness of stem cell therapy in Parkinson’s disease (PD) using PET imaging of substantia nigra with F-18-dihydrotetrabenazine (F-18-DTBZ), which has a high binding affinity (nm) for vesicular monoamine transporter 2.

Methods: PD was induced in normal adult, male rats by unilateral intracerebral injection of the dopamine neurotoxin 6-hydroxydopamine (Sigma; 20µg in 4µl PBS) into right median forebrain bundle. Motor function was evaluated at 3 and 6 weeks intervals following amphetamine (5mg/kg, i.p.) challenge and F-18-DTBZ PET imaging was performed (MOSAIC, Phillips). Rats with a motor deficit (rotational score ≥ 50/5 min, automated rotometer system) and lacking F-18-DTBZ uptake in the striatum ipsilateral to the lesion were surgically implanted either with 1x106 differentiated human embryonic stem cells (Wicell H9 line) or with 5x105 14d mouse fetal substantia nigra cells. Rats were monitored for motor function and periodical F-18-DTBZ PET imaging was performed. Normal striatum to treated striatum and striatum to cerebellum F-18 uptake ratios were calculated.

Results: The F-18-DTBZ uptake in normal striatum was 2.6 ± 0.2 times greater than cerebellum. In PD rats (≥ 50 rotations/5 min), this uptake was only 1.05 ± 0.05. The F-18 uptake in normal striatum was >2 in that of the lesioned striatum. The longitudinal studies to determine the effectiveness of stem cell therapy as determined by measurements of striatum uptake vs rotational score are in progress.

Conclusions: PET imaging with F-18-DTBZ provides a promising tool to monitor the effectiveness of stem cell therapy in PD.





This Article
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Right arrow Articles by Thakur, M.
Right arrow Articles by Iacovitti, L.
PubMed
Right arrow Articles by Thakur, M.
Right arrow Articles by Iacovitti, L.