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J Nucl Med. 2008; 49 (Supplement 1):151P
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Instrumentation & Data Analysis: Data Analysis & Management

Cardiac/Small Animal

Quantification of myocardial blood flow in rat myocardium with N-13-ammonia and a new microPET scanner

Marc Lamoureux1, Stephanie Thorn1, Ran Klein1, Mireille Lortie1, Jennifer Renaud1, Rob Beanlands1, Jean DaSilva1 and Robert deKemp1

1 University of Ottawa Heart Institute, Ottawa, Ontario, Canada

600

Objectives: MicroPET imaging is increasingly used to characterize animal models of disease and their therapies. This study aimed to quantitatively evaluate global and regional uniformity of myocardial blood flow (MBF) in normal rat myocardium using a Siemens Inveon PET scanner.

Methods: Six male Sprague Dawley rats (400-600g) were anesthetized (2% isoflurane) and iv-injected with 75-130 MBq of N-13-ammonia and PET data was acquired for 30 minutes. Dynamic images were reconstructed using 12x10s, 3x60s, 5x300s frames using OSEM3D/MAP without scatter or attenuation correction. FlowQuant © automated analysis software was then used to produce polar maps of regional MBF (ml/min/g) in the LV myocardium using a one-compartment model and the first two minutes of data. The polar maps were divided into five segments (anterior, posterior, lateral, septal and apical) and a one-way ANOVA tested for significant differences between segments.

Results: The mean MBF value in this population was determined to be (5.73±0.94) ml/min/g (mean ± SD), representing a 16% population variability consistent with previous experience in humans and canines. The mean regional MBF polar map showed notable uniformity, with a regional coefficient of variation (SD/mean) of 11%. A one-way ANOVA revealed no significant differences in MBF values between the five segments (p=0.23) further demonstrating the expected uniformity of the MBF polar maps of the normal rat myocardium.

Conclusions: A uniform MBF distribution, small population variability and flow values in the expected normal range were demonstrated. These results strongly suggest this system is suitable for quantitative PET imaging studies of MBF in rats.

Research Support: CIHR, NSERC, HSFO Program Grant, CFI/ORF.





This Article
Services
Right arrow Email this article to a friend
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Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Google Scholar
Right arrow Articles by Lamoureux, M.
Right arrow Articles by deKemp, R.
PubMed
Right arrow Articles by Lamoureux, M.
Right arrow Articles by deKemp, R.