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J Nucl Med. 2008; 49 (Supplement 1):133P
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General Clinical Specialties: Infectious Disease/Hematology

Infectious Disease/Hematology

Human leukocyte labeling with 64Cu: An intraindividual comparison with 111In-oxine & 18F-FDG

Kuldeep Bhargava1, Raj Gupta2, Charito Love1, Kenneth Nichols1 and Christopher Palestro1

1 Nuclear Medicine and Molecular Imaging, North Shore Long Island Jewish Health System, New Hyde Park, New York; 2 Physiology, Albert Einstein College of Medicine, Bronx, New York

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Objectives: To assess the feasibility of in-vitro leukocyte (WBC) labeling with 64Cu, comparing labeling efficiency, stability & viability to WBC's labeled with 111In-oxine (InWBC) & 18F-FDG (FDGWBC).

Methods: WBCs from 10 volunteers were labeled with 64Cu, 111In oxine, & 18F-FDG. 111In-oxine & 18F-FDG labelings were performed according to published methods. For 64Cu labeling, after red cell separation, WBCs were isolated from plasma by centrifugation at 450G for 10 min, washed with saline, incubated in 5 mL saline with 100 {upsilon}mole/L quin-MF/AM & 74-185 MBq 64Cu-tropolone for 45 min at 37\#9675;C. 5 mL platelet poor plasma (PPP) was added & the mixture centrifuged at 450 g for 5 min. Supernatant was removed & cells resuspended in 5 mL PPP. Labeling efficiency, label stability up to 24 hrs, & cell viability (using trypan blue exclusion test) up to 24 hrs were compared.

Results: Labeling efficiency was significantly higher for InWBC (86±4%), & CuWBC (87±4%) than for FDGWBC (60±19%,p<0.001). Label stability was significantly higher for InWBC than for CuWBC & FDGWBC up to 4 hrs (p < 0.001) & significantly higher than CuWBC at 24 hrs (p<0.005). Cell viability was significatly higher for CuWBC than InWBC & FDGWBC at 3 & 24 hrs (p<0.001).


Figure 1

Conclusions: 64Cu labels human WBC's with an efficiency similar to 111In-oxine & a significantly higher cell viability. Despite significantly lower stability than InWBC, CuWBC is a potentially useful PET infection imaging agent.





This Article
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Right arrow Articles by Palestro, C.